Part:BBa_K568005:Design

Intermediate optogenetical AND-Gate without T7ptag

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  INCOMPATIBLE WITH RFC[12]

  Illegal NheI site found at 7
  Illegal NheI site found at 30
  
• 21
  INCOMPATIBLE WITH RFC[21]

  Illegal BamHI site found at 644
  Illegal XhoI site found at 1786
  
• 23
  COMPATIBLE WITH RFC[23]
• 25
  INCOMPATIBLE WITH RFC[25]

  Illegal NgoMIV site found at 971
  Illegal AgeI site found at 3817
  
• 1000
  INCOMPATIBLE WITH RFC[1000]

  Illegal BsaI site found at 3820
  

Design Notes

This part was constructed in order to add T7ptag (BBa_K228000) at 3' site of intermediate construct in a next step.

Source

The red light sensor (BBa_K568000) was added to an intermediated part of the optogenetic AND gate (BBa_K568006).